A 20-minute assay for COVID-19 diagnosis



coronavirus , COVID-19
Image of the ultrastructural morphology exhibited by the 2019 Novel Coronavirus (2019-nCoV). Credit: CDC

Researchers have developed a brand new take a look at that may diagnose COVID-19 in simply 20 minutes.

The findings, printed within the Journal of Medical Microbiology, present the fast molecular known as N1-STOP-LAMP, is 100% correct in diagnosing samples containing SARS-CoV-2 at excessive masses.

The take a look at is extremely correct and simple to make use of, making it a for use in settings with restricted testing capabilities. The technique includes utilizing a small moveable machine, which may reliably detect SARS-CoV-2 from only one nasal swab. “In the race to control the COVID-19 pandemic, access to rapid, precision diagnostics is key. We have developed an alternative COVID-19 molecular test that can be readily deployed in settings where access to standard laboratory testing is limited or where ultra-rapid result turnaround times are needed” stated University of Melbourne Professor Tim Stinear, Laboratory Head on the Doherty Institute.

This makes use of just one tube and includes solely a single step, making it extra environment friendly and decrease value than most of the present exams for SARS-CoV-2. The N1-STOP-LAMP technique was discovered to be 100% correct and appropriately recognized 87% of exams as optimistic when used to evaluate 157 confirmed-positive samples. The outcomes had been quick, with a median time-to-positive of 14 minutes for 93 of these scientific samples.

“We see this kind of technology having benefit in settings liked aged care facilities, or overseas laboratories with and equipment,” Professor Stinear stated. “The test requires a small shoebox-sized machine, as well as reagents, but everything is portable.”

“STOP-LAMP is what’s referred to as a ‘near care’ test, it is not intended to replace the current gold standard PCR testing. It’s a robust diagnostic test for the specific and rapid detection of COVID-19. But it’s important to note however, it trades some detection sensitivity for speed and ease-of-use.”

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More data:
Jean Y. H. Lee et al. Validation of a single-step, single-tube reverse transcription loop-mediated isothermal amplification assay for fast detection of SARS-CoV-2 RNA, Journal of Medical Microbiology (2020). DOI: 10.1099/jmm.0.001238

Provided by
Microbiology Society

A 20-minute assay for COVID-19 diagnosis (2020, August 13)
retrieved 13 August 2020
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